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AKT1 Mutation Analysis

AKT1 Mutation Analysis

Indication:  

To detect and type mutations in exons 3 and 6 of the AKT1 oncogene in a wide range of human malignancies, including colorectal, ovarian, and breast cancers, for prognostic and therapeutic applications.

Methodology:  

This test is performed by PCR-based Sanger sequencing of DNA for codons 16 to 58 in exon 3 and codons 146 to 186 in exon 6 of the AKT1 gene.

Test Parameters:

This assay will detect mutations present in exons 3 and 6 of AKT1. The sensitivity of the Sanger sequencing assay is 20% of variant sequence in the background of wild-type sequence.

Turnaround Time:

7-10 days

Sample Requirements:
10 mL peripheral blood, 2-5mL bone marrow aspirate, 5g purified DNA, or 4-10 unstained tissue sections on glass slides along with 1 H&E-stained section.
Please provide a copy of the corresponding pathology report.

CPT Codes:

81479

Additional charges may apply for tissue extraction.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.


© 2014 The University of Texas MD Anderson Cancer Center