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KIT Mutation Detection in Sarcomas

Sequencing

Indication

To detect mutations in the KIT receptor tyrosine kinase, which are commonly present in gastrointestinal stromal tumors (70-80% of all cases). Mutations in KIT can be used to identify patients that may respond to tyrosine kinase inhibitors, as well as patients that acquire secondary mutations and develop resistance to therapy.

Methodology

PCR-based DNA sequencing.  This test also available on Next Gen Sequencing panel.

Test Parameters

The presence of mutations in selected codons of exons 2, 9, 10, 11, 13, 14, 15, 17 and 18 of KIT is assessed by PCR-DNA sequencing reaction. 

Sensitivity

The lower limit of sensitivity of detection is approximately one mutated cell per five total cells in sample (20%).

Turnaround Time

10-14 working days

Sample Requirements

  • 10 ug of purified DNA, sent on dry ice

    or
  • Four to six unstained recut slides of formalin-fixed, paraffin embedded tissue containing adequate amounts of tumor to be analyzed (See Sensitivity.)

The area of tumor to be analyzed should be indicated by circling the area on the bottom side of the slide or in a separate H&E-stained guide section.

Please provide a copy of the corresponding pathology report.

CPT Codes

81404

Additional charges will apply for tissue extraction.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.


© 2014 The University of Texas MD Anderson Cancer Center