KIT Mutation Detection in Sarcomas
Sequencing
Indication
To detect and type the activating point mutations in the KIT receptor tyrosine kinase, which are commonly present in gastrointestinal stromal tumors (70-80% of all cases).
Methodology
PCR-based DNA sequencing.
Test Parameters
The presence of mutations in exon 9 and 11 of KIT is assessed in two separate PCR-DNA sequencing reactions, which is done by screening exon 11 first followed by exon 9 if no mutation is detected.
Sensitivity
The lower limit of sensitivity of detection is approximately one mutated cell per five total cells in sample (20%).
Turnaround Time
10-14 working days
Sample Requirements
- 10 ug of purified DNA, sent on dry ice
or - Four to six unstained recut slides of formalin-fixed, paraffin embedded tissue containing adequate amounts of tumor to be analyzed (See Sensitivity.)
The area of tumor to be analyzed should be indicated by circling the area on the bottom side of the slide or in a separate H&E-stained guide section.
Please provide a copy of the corresponding pathology report.
CPT Codes
83890 (DNA Isolation), 83898 x 1 (for exon 11, additional for exon 9), 83904 x 1 (for exon 11, additional for exon 9), 83912
Additional charges will apply for tissue extraction.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.