KIT Mutation Detection in Sarcomas
Sequencing
Indication
To detect mutations in the KIT receptor tyrosine kinase, which are commonly present in gastrointestinal stromal tumors (70-80% of all cases). Mutations in KIT can be used to identify patients that may respond to tyrosine kinase inhibitors, as well as patients that acquire secondary mutations and develop resistance to therapy.
Methodology
PCR-based DNA sequencing. This test also available on Next Gen Sequencing panel.
Test Parameters
The presence of mutations in selected codons of exons 2, 9, 10, 11, 13, 14, 15, 17 and 18 of KIT is assessed by PCR-DNA sequencing reaction.
Sensitivity
The lower limit of sensitivity of detection is approximately one mutated cell per five total cells in sample (20%).
Turnaround Time
10-14 working days
Sample Requirements
- 10 ug of purified DNA, sent on dry ice
or - Four to six unstained recut slides of formalin-fixed, paraffin embedded tissue containing adequate amounts of tumor to be analyzed (See Sensitivity.)
The area of tumor to be analyzed should be indicated by circling the area on the bottom side of the slide or in a separate H&E-stained guide section.
Please provide a copy of the corresponding pathology report.
CPT Codes
81404
Additional charges will apply for tissue extraction.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.