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JAK2-Exon 12 Mutational Analysis


Although most cases of polycythemia vera (PV) has the p.V617F activating mutation in the JAK2 tyrosine kinase, a small proportion of PV cases may have mutations (usually deletions) in exon 12.


PCR-based Sanger sequencing of DNA is performed to examine mutational status of exon 12 of JAK2.

Test Parameters

Tumor clones must comprise at least 20% of the cells in the sample for accurate assessment.

Turnaround Time

10 days

Sample Requirements

  • 10 ml peripheral blood in purple top tube (EDTA Vacutainer), sent on wet ice

  • 2-5 ml of bone marrow aspirate, sent on wet ice

  • Frozen tissue samples

  • Formalin-fixed, paraffin embedded tissue blocks

Please provide a copy of the corresponding pathology report.

CPT Codes


Additional charges will apply for tissue extraction.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

© 2015 The University of Texas MD Anderson Cancer Center