hMLH1 Methylation Status
Indication
For assessment of hMLH1 promoter methylation status in patients with colorectal cancer, where hMLH1 expression is lacking, or in which MSI+ status has been documented.
Methodology
Bisulfite treatment of DNA followed by PCR amplification of both unmethylated and methylated hMLH1 promoter sequences with products detected by capillary electrophoresis / GeneScan analysis.
Test Parameters
Detection of methylation in a small proximal region of the gene promoter only. Partial methylation may not be detectable due to limitation of the methodology.
Turnaround Time
Eight to 10 working days
Sample Requirements
10-µm sections of microdissected, paraffin-embedded tissue on five to six slides with separation or indication of normal and tumor areas. Send at room temperature.
Please provide a copy of the corresponding pathology report.
CPT Codes
83890 (DNA Isolation), 83900, 83909, 83912
Additional charges may apply for tissue extraction.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.