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Cyclin D1/BCL1/t (11;14) Gene Rearrangement (PCR)

Indication

For determining the presence or absence of the rearranged BCL1 gene in newly presenting cases of mantle cell or unclassified lymphoma or for monitoring for minimal residual disease.

Methodology

DNA is analyzed for IGH/BCL1 fusions involving the major translocating cluster (MTC) region of the BCL1 gene by quantitative real-time PCR using the tagman method.

Test Parameters

This assay will only detect JH/BCL1 rearrangements in the 30-40% of mantle cell lymphoma cases that have translocations involving the MTC region. Conventional karyotyping and FISH studies can be used to evaluate for JH/BCL1 rearrangements outside of this region. The sensitivity of the test is reported with each assay but is approximately one t(11;14)-bearing cell per 10,000 total cells.

Turnaround Time

Five to 10 working days

Sample Requirements

  • 10 ml peripheral blood in purple top tube (EDTA Vacutainer), sent by overnight express mail on wet ice

    or
  • 2-5 ml of bone marrow aspirate in purple top tube (EDTA Vacutainer), sent by overnight express mail, sent on wet ice

    or
  • 10 µg of purified DNA, sent by overnight express mail on dry ice, if available

CPT Codes

81401

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.


© 2014 The University of Texas MD Anderson Cancer Center