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Chimerism Assessment by Polymorphic Microsatellite Markers

Pre-transplant Screening

Indication

- To evaluate and monitor engraftment after hematopoietic stem cell transplant using total cells (both peripheral blood and bone marrow) and ,sorted T- and myeloid cells (bone marrow only). Detection of microsatelllite markers that are exclusively donor in origin indicates successful engraftment. Serial values showing a decline i engraftment of donor cells may predict a graft-failure in appropriate clinical settings.

- To identify the source of successful engraftment when more than one donors are used from the hematopoietic stem cell transplant.

Methodology

Initial screening of pre-transplant and donor samples involves PCR for eight microsatellite markers to identify an informative marker that can be used for subsequent analysis.

Test Parameters

Peripheral blood samples are required from both donor and recipient.

Turnaround Time

Five to seven working days

Sample Requirements

  • 10 ml peripheral blood in purple top tube (EDTA Vacutainer), sent on wet ice

    or
  • 2-5 ml of bone marrow aspirate, sent on wet ice

CPT Codes

81265, 81267, 81268

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.


© 2014 The University of Texas MD Anderson Cancer Center