Chimerism Assessment by Polymorphic Microsatellite Markers
Pre-transplant Screening
Indication
- To evaluate and monitor engraftment after hematopoietic stem cell transplant using total cells (both peripheral blood and bone marrow) and ,sorted T- and myeloid cells (bone marrow only). Detection of microsatelllite markers that are exclusively donor in origin indicates successful engraftment. Serial values showing a decline i engraftment of donor cells may predict a graft-failure in appropriate clinical settings.
- To identify the source of successful engraftment when more than one donors are used from the hematopoietic stem cell transplant.
Methodology
Initial screening of pre-transplant and donor samples involves PCR for eight microsatellite markers to identify an informative marker that can be used for subsequent analysis.
Test Parameters
Peripheral blood samples are required from both donor and recipient.
Turnaround Time
Five to seven working days
Sample Requirements
- 10 ml peripheral blood in purple top tube (EDTA Vacutainer), sent on wet ice
or - 2-5 ml of bone marrow aspirate, sent on wet ice
CPT Codes
81265, 81267, 81268
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.