t(9;22)(q34;q11.2);BCR-ABL1, Quantitative Transcript Analysis
Real-Time and Nested PCR
Detection of the t(9;22)(q34;q11.2);BCR-ABL1 fusion transcript for diagnosis and monitoring of residual disease in patients with chronic myelogenous leukemia (CML), B-lymphoblastic leukemia (B-ALL) with t(9;22) and a small subset of patients with acute myeloid leukemia (AML).
RNA is extracted from white blood cells in bone marrow and/or peripheral blood and reverse transcribed to cDNA. Real-time PCR is performed to amplify the BCR-ABL transcripts as well as ABL transcripts. Transcript types b3a2, b2a2 and e1a2 are detected in single tube reaction.
Sensitivity is approximately one in 100,000 for real-time PCR. Levels detected in peripheral blood and bone marrow samples are generally equivalent.
Five to 10 working days
- 10-30 ml peripheral blood in lavender top (EDTA) tube, sent on wet ice
- 2-5 ml of bone marrow aspirate in lavender top (EDTA) tube, sent on wet ice
81206, 81207, 81208
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