t(9;22)(q34;q11.2);BCR-ABL1, Quantitative Transcript Analysis
Real-Time and Nested PCR
Indication
Detection of the t(9;22)(q34;q11.2);BCR-ABL1 fusion transcript for diagnosis and monitoring of residual disease in patients with chronic myelogenous leukemia (CML), B-lymphoblastic leukemia (B-ALL) with t(9;22) and a small subset of patients with acute myeloid leukemia (AML).
Methodology
RNA is extracted from white blood cells in bone marrow and/or peripheral blood and reverse transcribed to cDNA. Real-time PCR is performed to amplify the BCR-ABL transcripts as well as ABL transcripts. Transcript types b3a2, b2a2 and e1a2 are detected in single tube reaction.
Test Parameters
Sensitivity is approximately one in 100,000 for real-time PCR. Levels detected in peripheral blood and bone marrow samples are generally equivalent.
Turnaround Time
Five to 10 working days
Sample Requirements
- 10-30 ml peripheral blood in lavender top (EDTA) tube, sent on wet ice
or - 2-5 ml of bone marrow aspirate in lavender top (EDTA) tube, sent on wet ice
CPT Codes
81206, 81207, 81208
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.