t(9;22)(q34;q11.2);BCR-ABL1, Quantitative Transcript Analysis
t(9;22)(q34;q11.2);BCR-ABL1 Analysis by PCR
Real-Time (Quantitative), Nested PCR (Qualitative)
Detection of the t(9;22)(q34;q11.2);BCR-ABL1 fusion transcript for diagnosis and monitoring of residual disease in patients with chronic myelogenous leukemia (CML), B-lymphoblastic leukemia (B-ALL) with t(9;22) and a small subset of patients with acute myeloid leukemia (AML).
RNA is extracted from white blood cells in bone marrow and/or peripheral blood and reverse transcribed to cDNA. Real-time PCR is performed to amplify the BCR-ABL1 fusion transcripts as well as ABL1 transcripts. Common transcript types also known as major (e13a2 (b2a2), e14a2 (b2a2)) and minor (e1a2) are detected in single tube reaction by real-time Quantitative PCR. When an Alternate transcript (e13a3 (b2a3), e14a3 (b3a3) and e19a3) is suspected, a qualitative nested PCR is performed, followed by capillary electrophoresis and Sanger sequencing for identification of fusion transcript.
Sensitivity is approximately 1 in 100,000 for real-time PCR and 1in 100,000 for qualitative nested PCR. Levels detected in peripheral blood and bone marrow samples are generally equivalent.
5-10 working days (Quantitative); 5-15 working days (Qualitative).
• 10-30 ml peripheral blood in lavender top (EDTA) tube, sent on wet ice
• 2-5 ml of bone marrow aspirate in lavender top (EDTA) tube, sent on wet ice
81206, 81207, 81208
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