AML1-ETO/t(8;21) Fusion Transcript Detection
Quantitative RNA-PCR
Indication
For determining the presence or absence of the AML1-ETO fusion transcript that is associated with the t(8;21) chromosomal abnormality seen in a subset of AML-M2 cases. This quantitative test can be used for diagnosis or for monitoring levels of minimal residual disease detection following therapy.
Methodology
RNA is analyzed for AML1-ETO fusion by reverse transcription followed by quantitative real-time PCR using the TaqMan method.
Test Parameters
This assay will detect AML1-ETO fusion transcripts. Values are expressed as a percentage of AML1-ETO to normalizing ABL transcripts. The sensitivity of detection is approximately one in 100,000.
Turnaround Time
Five to 10 working days
Sample Requirements
- 10 ml peripheral blood in purple top tube (EDTA Vacutainer), sent by overnight express mail on wet ice
or - 2-5 ml of bone marrow aspirate in purple top tube (EDTA Vacutainer), sent by overnight express mail, sent on wet ice
or - 10 µg of purified RNA or cDNA, sent by overnight express mail on dry ice.
CPT Codes
83891 (RNA isolation), 83902, 83898, 83912
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.