18q Loss of Heterozygosity (LOH)
Indication
Used as a prognostic marker in colorectal cancer or carcinomas from other sites.
Methodology
Multiplex PCR of the following markers: D18S55, D18S58, D18S61, D18S64 and D18S69. Alleles ratio calculated from capillary electrophoresis data of the five markers is then used to assess LOH.
Test Parameters
Both samples from tumor and normal (uninvolved) samples must be analyzed to assess for loss of heterozygosity.
Turnaround Time
Seven to 10 working days
Sample Requirements
- Sections of microdissected, paraffin-embedded tissue fixed on five to six slides (with clear indication of tumor and normal areas)
or - Frozen tissue samples (separated into normal and tumor)
Please provide a copy of the corresponding pathology report.
CPT Codes
83890 (DNA Isolation), 83900, 83909, 83912
Additional charges will apply for tissue extraction.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.