Molecular Cytogenetics Facility
Dr. Sen Pathak, Dr. Asha Multani, and Jin Ma
The T. C. Hsu Molecular Cytogenetics Facility (MCF) is funded by the Center for Genetics and Genomics (CGG). Dr. Guillermina Lozano is the Director and Dr. Asha S. Multani is the Co-Director of the facility. The MCF is administered through the Department of Genetics.
This facility offers Conventional as well as Molecular Cytogenetic services including cell line authentication, species identification, karyotyping, analysis of genomic instability, fluorescence in situ hybridization (Figure 1) and Spectral Karyotyping (Figure 2).
One of the key expertise of our facility is to validate the origin of cell lines. Approximately 20-30% of all cell lines used in biomedical research are contaminated (1,2). Many top tier journals have begun to request proof of cell line authentication from investigators. Unlike other methods, chromosomal analysis is able to unambiguously identify cells from all common mammalian species and can also differentiate between cells from the same species. This analysis can therefore identify intra-species and inter-species cell line contaminations.
Figure 1: Telomere fluorescence in situ hybridization (FISH) in mouse metaphase
1. Bravo NR, Gottesman M. Notice Regarding Authentication of Cultured Cell Lines. 2007.
Figure 2: Spectral karyotyping (SKY) of mouse chromosomes
2. Chatterjee R. Cell biology. Cases of mistaken identity. Science 315(5814):928-31, 2007.
Please contact Dr. Multani for guidelines for sample preparation, technical assistance and to discuss the services that you require.
Asha S. Multani, Ph.D.
We will take the time to understand your project and plan for the preparation of samples for analysis. We will help with interpretation of the results and upon request provide a detailed description of method as well as microphotographs (color or black and white) suitable for inclusion in reports or manuscripts to the investigators.
Center Grant Citation
Publications should cite the Center for Genetics and Genomics in the acknowledgment section if publications use data generated by this Core facility. Two copies of the publication acknowledging the Core funding should also be submitted to the facility at 1515 Holcombe Blvd., Unit 1010, Houston, TX, 77030.
Gallardo M, Lee HJ, Zhang X, Bueso-Ramos C, Pageon LR, McArthur M, Multani A, Nazha A, Manshouri T, Parker-Thornburg J, Rapado I, Qunitas-Cardama A, Kornblau SM, Martinez-Lopez J, Post SM. hnRNP K is a novel haploinsufficient tumor suppressor that regulates proliferation and differentiation programs in hematologic malignancies. Cancer Cell. pii: S1535-6108(15)00305-0. 9/2015 PMID: 26412324
Press Release: MD Anderson study identifies leukemia tumor suppressor
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