Services
Services provided by the Immune-Monitoring Core Laboratory include:
- Consultation on experimental and clinical trial design involving immune monitoring
- EliSpot assays (e.g., IFN alpha, IL-4, GRANZYME B)
- Leukocyte phenotype analysis with FACS (1-6 color)
- Intracellular cytokine staining (IFN alpha, TNF-a)
- HLA Tetramer/pentamer staining
- Cell proliferation assays (MTT, CFSE)
- Multiplex cytokine assays using Luminex-100 System
- New sensitive flow cytometry-based CTL assay using caspase 3-cleavage in Target cells as a readout
- Processing and storage of PBMC samples from clinical trials (on a case-by-case basis)
- TCR Vβ Clonotype analysis (in collaboration with Dr. Jeff Molldrem)
- TCR gene excision circle (TREC) analysis for circulating Naïve T-cell (in collaboration with Dr. Krishna Komanduri)