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Services and Fees

Gene Targeting via Electroporation

  • Electroporation includes picking of all colonies surviving selection up to 288 clones (3 x 96-well plates) and expansion of up to three clones. Germline transmission is not guaranteed
  • Fee: $4000/construct for MD Anderson investigators; $10,160/construct for MD Anderson-affiliated investigators; $16,256/construct for other outside investigators

Picking of 96-Well Plates

  • Additional 96-well plates picked
  • Fee: $250/plate for MD Anderson investigators; $550/plate for external investigators

Colonies from 96-Well Plates

  • ES cell expansion of additional colonies from 96-well plates
  • Fee: $250/clone for MD Anderson investigators; $550/clone for external investigators

ES Cell Expansion and Cytogenetic Analysis

  • Prior to injection of cells generated by the Knockout Consortium, they must be expanded and checked cytogenetically.
  • Fee: $1000/clone for MD Anderson investigators;  $2700/clone for MD Anderson-affiliated investigators; $4320/clone for other outside investigators

Generation of ES Cells from Blastocysts

  • ES cell generation will include the isolation of the inner cell mass and subsequent growth ES cells from blastocysts obtained from five to 10 superovulated females
  • Fee: $4000 for MD Anderson investigators; $10,160 for MD Anderson-affiliated investigators; $16,256/construct for other outside investigators

Blastocyst Injections and Morula Aggregations

  • Blastocyst injections and morula aggregations include injection of 50 blastocysts or aggregation of up to 50 morulae for all cell lines. For cell lines produced by the GEMF, we will substitute an additional clone (if available) for a clone that does not produce chimeras
  • Fee: $1400/clone for MD Anderson investigators; $3500/clone for MD Anderson-affiliated investigators; $5600/clone for other outside investigators

CRISPR/Cas9 Gene Targeting by Pronuclear Injection

  • The GEMF will inject sgRNA and Cas9 mRNA or protein into pronuclear stage mouse embryos. Components must be generated by an approved vendor or by the GEMF. A minimum of five injection days will be scheduled for CRISPR/Cas9 targeting. We cannot guarantee correct targeting, but will inject and implant a minimum of 240 embryos.
  • Fee Schedule:
Type of InvestigatorGEMF Isolation of Injection ComponentsApproved Vendor Isolation of Injection Components
MD Anderson investigators$2150$1850
MD Anderson-affiliated investigators$5400$4720
Other outside investigators$8640$7552


Standard Pronuclear Injections

  • Pronuclear injections include a guarantee of three founders for constructs injected into C57BL/6, FVB and B6D2F1 strains with proof of single copy detection prior to injection. For BAC transgenics, we only guarantee the injection of 200 embryos; there is no founder guarantee for BAC transgenics
  • Fee: $1850/construct for MD Anderson investigators; $4720/construct for MD Anderson-affiliated investigators; $7552/construct for other outside investigators

Embryo Cryopreservation

  • Embryo Cryopreservation: We will cryopreserve 200 2-cell embryos for heterozygous males mated to wild type females (for those strains amenable to this procedure) or 100 2-cell embryos for homozygous males mated to wild type or homozygous females. We generally average 15 good morulae from a C57BL/6 or hybrid, non-transgenic, superovulated female that is mated to a fertile male. Due to increased mouse costs, investigators will be expected to purchase females for projects where the background strain is not maintained in-house.
  • Fee: $925/line/session for MD Anderson investigators; $1900/line/session for MD Anderson-affiliated investigators; $3040/line/session for other outside investigators

Sperm Cryopreservation

  • Sperm Cryopreservation: We will freeze a minimum of ten and a maximum of 25 straws of sperm for each line. You will need to supply two to four males that have undergone previous mating, and that are about 6 months of age. All frozen sperm is tested by IVF to assure competency after thaw
  • Fee: $925/line/session for MD Anderson investigators; $1900/line/session for MD Anderson-affiliated investigators; $3040/line/session for other outside investigators

IVF-Cryopreservation

  • IVF-Cryopreservation: We will vitrify 200 two-cell embryos from IVF done with sperm from a heterozygous male, and 100 two-cell embryos from IVF done with sperm from a homozygous male. Due to increased mouse costs, investigators will be expected to purchase females for projects where the background strain is not maintained in-house. Please note: Waiting time for this procedure may be long
  • Fee: $925/line/session for MD Anderson investigators; $1900/line/session for MD Anderson-affiliated investigators; $3040/line/session for other outside investigators

Rederivation (Standard Procedure)

  • Rederivation: We will mate homozygotes to obtain embryos for homozygous lines, or mate homozygous or heterozygous males to wild type females for lines that may be rederived as heterozygotes.
  • Fee: $925/line/session for MD Anderson investigators; $2330/line/session for MD Anderson-affiliated investigators; $3728/line/session for other outside investigators

Embryo Implantation

  • Embryo Implantation: We will implant embryos (frozen or fresh) into pseudopregnant females for gestation.  Animals resulting will be transferred to the investigator for genotyping and breeding.
  • Fee:  $570 for MD Anderson investigators; $1440 for MD Anderson-affiliated investigators; $2304 for other outside investigators

In Vitro Fertilization

  • In Vitro Fertilization: We will use fresh or previously frozen sperm to fertilize oocytes obtained from up to 10 superovulated females. Embryos surviving to the two-cell stage will be implanted into pseudopregnant females for gestation or frozen for later implant. All surviving pups will be transferred to the contracting investigator. Due to increased mouse costs, investigators will be expected to purchase females for projects where the background strain is not maintained in-house
  • Fee: $1350 for MD Anderson investigators; $3410 for MD Anderson-affiliated investigators; $5456 for other outside investigators

Mouse Resource Facility

Services and Fees

Mouse Resource Facility services and fees are as follows:

  • Mice: $35/mouse (MD Anderson investigators only)
  • Excess stock animals (wild type): $15/mouse (MD Anderson investigators only)
  • Northern filter: $30/use for MD Anderson investigators; $60/use for external investigators
  • Straw of frozen embryos: $50/straw for MD Anderson investigators; $100/straw for external investigators
  • Hormones (PMS and HCG): $20/set for MD Anderson investigators; $40/set for external investigators
  • Embryo flushing and packing for shipping: $30 for MD Anderson investigators; $60 for external investigators
  • M15 media: $30/50 ml for MD Anderson investigators; $60/50 ml for external investigators

Additional Services

  • Additional charges for fees charged to the GEMF by VetMed (for animals maintained in the GEMF past weaning, technical fees for superovulation and mating procedures, packaging and shipping fees or fees for health certificates) will be allocated to the appropriate PI as necessary
  • Fee: at cost

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For questions or assistance, contact Jan Parker-Thornburg at jpthorn@mdanderson.org or Katherine Hale at khale@mdanderson.org.


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