Services and Fees

Electroporations

• Electroporation includes picking of all colonies surviving selection up to 288 clones (3 x 96-well plates) and expansion of up to three clones. Germline transmission is not guaranteed
• Fee: $4000/construct for MD Anderson investigators; $10,900/construct for external investigators
• Available at both North and South Campus sites

Picking of 96-Well Plates

• Additional 96-well plates picked
• Fee: $250/plate for MD Anderson investigators; $550/plate for external investigators
• Available at both North and South Campus sites

Colonies from 96-Well Plates

• ES cell expansion of additional colonies from 96-well plates
• Fee: $250/clone for MD Anderson investigators; $550/clone for external investigators
• Available at both North and South Campus sites

Generation of ES Cells from Blastocysts

• ES cell generation will include the isolation of the inner cell mass and subsequent growth ES cells from blastocysts obtained from five to 10 superovulated females
• Fee: $4000 for MD Anderson investigators; $10,900 for external investigators

Blastocyst Injections and Morula Aggregations

• Blastocyst injections and morula aggregations includes injection of 50 blastocysts or aggregation of up to 50 morulae for all cell lines. For cell lines produced by the GEMF, we will substitute an additional clone (if available) for a clone that does not produce chimeras
• Fee: $1500/cell line for MD Anderson investigators; $4090/cell line for external investigators
• Available at both North and South Campus sites

KOMP/EUCOMM Blastocyst Injections

• KOMP/EUCOMM blastocyst injections includes expansion, cytogenetic testing, and injection of 100 blastocysts
• Fee: $6000/cell line for MD Anderson investigators; $16,000/cell line for external investigators
• Available at both North and South Campus sites

Pronuclear Injections

• Pronuclear injections includes a guarantee of three founders for constructs injected into C57BL/6, FVB and B6D2F1 strains with proof of single copy detection prior to injection. For BAC transgenics, we only guarantee the injection of 200 embryos; there is no founder guarantee for BAC transgenics
• Fee: $1500/construct for MD Anderson investigators; $4090/construct for external investigators

Standard Embryo Cryopreservation

• Standard Embryo Cryopreservation: We will cryopreserve 200 embryos for heterozygous males mated to wild type females at morula stage (for those strains amenable to this procedure) or 100 embryos for homozygous males. We generally average 15 good morulae from a C57BL/6 or hybrid, non-transgenic, superovulated female that is mated to a fertile male. Due to increased mouse costs, investigators will be expected to purchase females for projects where the background strain is not maintained in-house. We will apply a 10% discount for supplying proven male breeders to be demonstrated as follows:
  • Age = 3-6 months
  • Breeding records indicating two recent litters of more than six pups
• Fee: $1100/line/session for MD Anderson investigators; $3000/line/session for external investigators

Sperm Cryopreservation

• Sperm Cryopreservation: We will freeze a minimum of five and a maximum of 25 straws of sperm for each line. You will need to supply two to four males that have undergone previous mating, and that are about 6 months of age. All frozen sperm is tested by IVF to assure competency after thaw
• Fee: $730/line for MD Anderson investigators; $2000/line for external investigators

IVF-Cryopreservation

• IVF-Cryopreservation: We will vitrify 200 two-cell embryos from IVF done with sperm from a heterozygous male, and 100 two-cell embryos from IVF done with sperm from a homozygous male. Due to increased mouse costs, investigators will be expected to purchase females for projects where the background strain is not maintained in-house. Please note: Waiting time for this procedure may be long
• Fee: $2000/line for MD Anderson investigators; $5450/line for external investigators

Rederivation (Standard Procedure)

• Rederivation: We will apply a 10% discount for supplying proven male breeders to be demonstrated as follows:
  • Age = 3-6 months
  • Breeding records indicating two recent litters of more than six pups
• We will reduce this fee by half if the investigator provides oviducts from superovulated, mated females or frozen embryos for the procedure
• $700/session for MD Anderson investigators; $1910/session for external investigators

In Vitro Fertilization

• In Vitro Fertilization: We will use fresh or previously frozen sperm to fertilize oocytes obtained from five to 10 superovulated females. All embryos surviving to the two-cell stage will be implanted into pseudopregnant females for gestation. All surviving pups will be transferred to the contracting investigator. Due to increased mouse costs, investigators will be expected to purchase females for projects where the background strain is not maintained in-house
• Fee: $1125 for MD Anderson investigators; $3070 for external investigators

Mouse Resource Facility

Services and Fees

Mouse Resource Facility services and fees are as follows:

• Mice: $30/mouse (MD Anderson investigators only)
• Excess stock animals (wild type): $15/mouse (MD Anderson investigators only)
• Library: $30/aliquot for MD Anderson investigators; $60/aliquot for external investigators
• BAC filter: $30/use for MD Anderson investigators; $60/use for external investigators
• Northern filter: $30/use for MD Anderson investigators; $60/use for external investigators
• Straw of frozen embryos: $50/straw for MD Anderson investigators; $100/straw for external investigators
• Hormones (PMS and HCG): $20/set for MD Anderson investigators; $40/set for external investigators
• Embryo flushing and packing for shipping: $30 for MD Anderson investigators; $60 for external investigators
• M15 media: $30/50 ml for MD Anderson investigators; $60/50 ml for external investigators

Additional Services

• Additional charges for fees charged to the GEMF by VetMed (for animals maintained in the GEMF past weaning, technical fees for superovulation and mating procedures, packaging and shipping fees or fees for health certificates) will be allocated to the appropriate PI as necessary
• Fee: at cost