Genetically Engineered Mouse Facility

The purpose of the Genetically Engineered Mouse Facility (GEMF) at is to provide technologically advanced and efficient mouse mutation resources to faculty members at the institution. Modifications to the genome utilizing direct DNA injection and ES cell mutagenesis, cryopreservation, in vitro fertilization and rederivation of mouse lines are all technologies supported by the facility.

The facility is fully equipped with the latest instrumentation and staffed with highly skilled personnel trained specifically for the production of mutant mice.

Announcements

The GEMF is now freezing sperm utilizing the new Jackson Lab protocol. This is a very easy, cheap and reliable method to take your mice off the shelf, or to insure them against disease and genetic drift. You will need two to four fertile males (3-6 months old, and previously mated), and the cost will be $325. Please contact Jan Parker-Thornburg for the service request form and for additional information.
The GEMF now has BALB/c ES cells that can be used for targeting. We have tested and obtained germline transmission from BALB/c ES cells we received from Switzerland. While they are not as robust as hybrid or standard 129 cells, we have shown germline transmission in one out of four low percentage chimeras. For more information, please contact Jan Parker-Thornburg.
The GEMF also operates a satellite facility on South Campus, which provides ES cell electroporation and blastocyst injection. For more information, please contact Chen Dong at 713-563-3263.