Applying RPPA in my Studies
RPPA analyzes cellular protein activity in signaling networks and can be applied to normal or tumor tissue, and also to cultured cells.
Cell Lines
- Characterize cellular signaling networks under different culture conditions
- Determine drug selectivity
- Identify therapeutic targets
- Define regulatory mechanisms in signaling networks, including forward and feedback loops and cross talks
- Ability to analyze up to 1000 conditions concurrently and allows the detailed concentration and time courses required for systems biology approaches
Patient Samples
- Classify patient tumors
- Correlate DNA, RNA and Protein
- Determine prognosis
- Predict responses to targeted therapies
- Pharmacodynamics and biologically relevant dose
- Determine appropriate handling procedures for clinical samples (based on antigen stability analysis)