Services and Fees - South Campus
Analytical Flow Cytometry
Flow Cytometry is a tool in all aspects of cell biology research. Protein expression or indicators of cell status are simultaneously measured on each cell within a population. The Core provides access to cytometry equipment and staff with sufficient expertise to assist researchers in developing complex assays to address pressing research questions.
Cell Sorting
We isolate cells of a wide variety of different types based on many different combinations of Ab-based stains, fluorescent protein expression, & viability indicators.
BD Pathway 435 Confocal Imaging
This is a spinning disk confocal system equipped with laser and image autofocus, with a fixed stage & moving objective. A number of assays have been developed for this system, including apoptosis assays, Ca2+ flux assay, characterization of diffuse vs punctate staining in AML cells, assays of immune cell interactions, cell counts and transfection efficiency
Custom mAb conjugations
The Core performs conjugations primarily for mAb that are not commercially available as conjugates. Kathryn Ruisaard & Dr. Clise-Dwyer have more than 5 years experience doing microscale Ab conjugations for the Transplant Immunology section. Fluorochromes available include, Alexa 488, 647, 555, 594, Pacific Blue, and Pacific Orange.
Rates for MD Anderson Cancer Center Members
| Service | Assisted Rate | Unassisted Rate | Unit |
|---|---|---|---|
| Sorting | $65 | $35 | Hour |
| Analysis | $60 | $35 | Hour |
| Imaging | $60 | $45 | Hour |
| mAb Conjugations | $100 | N/A | 0.1mg Reaction |
