Services and Fees - South Campus
Analytical Flow Cytometry
Flow Cytometry is a tool in all aspects of cell biology research. Protein expression or indicators of cell status are simultaneously measured on each cell within a population. The Core provides access to cytometry equipment and staff with sufficient expertise to assist researchers in developing complex assays to address pressing research questions.
We use droplet based high-speed cell sorting to isolate cells of a wide variety of different types based on many different combinations of antibody-based stains, fluorescent protein expression, & viability indicators.
The Core has 2 Image Cytometry systems. The Amnis ImageStreamX MarkII is an imaging flow cytometer that uses CCD cameras to image cells in flow in up to 9 fluorescent channels for each cell. The Pathway 435 is a spinning disk confocal system equipped with laser and image autofocus, with a fixed stage & moving objective. A number of assays have been developed for both systems, including colocalization, nuclear translocation, apoptosis assays, Ca2+ flux assay, characterization of diffuse vs. punctate staining, assays of immune cell interactions, cell counts and transfection efficiency.
Custom mAb conjugations
The Core performs conjugations primarily for mAb that are not commercially available as conjugates. Kathryn Ruisaard and Dr. Clise-Dwyer have more than 5 years' experience doing microscale Ab conjugations for the Transplant Immunology section. Fluorochromes available include, Alexa 488, 647, 555, 594, Pacific Blue, and Pacific Orange. Contact Kathryn to schedule: 713-563-3350.
Rates for MD Anderson Cancer Center Members
|Service||Assisted Rate||Unassisted Rate||Unit|
|Analytical Flow Cytometry||$70||$40||Hour|
|mAb Conjugations||$100||N/A||0.1mg Reaction|