South Campus Equipment
Aria #1 Cell Sorter
Optics/Configurations: 3-laser, 9-color
Laser: 405
Filter 450/50BP
Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 530/30 BP
Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510
Laser: 488
Filter 530/30 BP
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 576/26 BP
PE, DS Red
Filter 610/20 BP
PE-Texas Red
Filter 695/40 BP
PE-Cy5, PerCP, PerCP-Cy5, PerCP-Cy5-5, PI, 7AAD
Filter 780/60 BP
PE-Cy7
Laser: 633
Filter 660/20 BP
APC, Alexa Fluor647, Sytox Red
Filter 780/60 BP
Alexa Fluor700, APC-CY7, APC-H7
Software Version: BD FACSDiva 6.1.3
Sample Tube: 12mmX75mm polystyrene tube
Sample Collection:
15ml tubes (up to two populations)
12mmX75mm tubes (up to four populations)
1.5ml microcentrifuge tubes (up to four populations)
24,96,384 well plates
Nozzle/pressure: 70um 70psi (default), 100um (by request; call flow staff for more information)
Aria #2 Cell Sorter
Optics/Configurations: 2-laser, 7-color
Laser: 488
Filter 530/30 BP
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 576/26 BP
PE, DS Red
Filter 610/20 BP
PE-Texas Red
Filter 695/40 BP
PE-Cy5, PerCP, PerCP-Cy5, PerCP-Cy5-5, PI, 7AAD
Filter 780/60 BP
PE-Cy7
Laser: 640
Filter 660/20 BP
APC, Alexa Fluor647, Sytox Red
Filter 780/60 BP
Alexa Fluor700, APC-CY7, APC-H7
Software Version: BD FACSDiva 6.1.3
Sample Tube: 12mmX75mm polystyrene tube
Sample Collection:
15ml tubes (up to two populations)
12mmX75mm tubes (up to four populations)
1.5ml microcentrifuge tubes (up to four populations)
Nozzle/pressure: 70um 70psi (default), 100um (by request; call flow staff for more information)
Influx Cell Sorter
Optics/Configurations: 5-laser, 14-color
Laser: UV355
Filter 460/50 BP
Hoechst Blue
Filter 670/30 BP
Hoechst Red
Laser: 405
Filter 460/50BP
Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 520/35 BP
Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510
Laser: 488
Filter 530/40 BP
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 670/30 BP
PerCP, PI, 7AAD
Filter 695/40 BP
PerCP-Cy5, PerCP-Cy5-5
Laser: 561
Filter 585/29 BP
PE, DS Red
Filter 610/20 BP
PE-Texas Red, Alexa Fluor610, mCherry, mKate, tdTomato
Filter 670/30 BP
PE-Cy5
Filter 750LP
PE-Cy7
Laser: 640
Filter 670/30 BP
APC, Alexa Fluor647, Sytox Red
Filter 720/40 BP
Alexa Fluor700
Filter 750 LP
APC-CY7, APC-H7
Software Version: BD Sortware 1.2
Sample Tube: 12mmX75mm polypropylene tube
Sample Collection:
15ml tubes (up to two populations)
12mmX75mm tubes (up to four populations)
50ml tube (up to two populations in combination with 1.5ml tube)
1.5ml microcentrifuge tubes (up to four populations)
24,96,384 well plates
Nozzle/pressure: 100um 24 psi (default), 70um 52psi (by request; call flow staff for more information)
Jazz Cell Sorter*
Optics/Configurations: 2-laser, 6-color
Laser: 488
Filter 530/40 BP
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 576/26 BP
PE, DS Red
Filter 629/40 BP
PerCP, PerCP-Cy5, PerCP-Cy5-5, PI, 7AAD
Filter 750 LP
PE-Cy7
Laser: 640
Filter 670/30 BP
APC, Alexa Fluor647, Sytox Red
Filter 750 LP
APC-CY7, APC-H7
Software Version: BD Sortware 1.2
Sample Tube: 5ml polypropylene tube
Sample Collection:
15ml tubes (up to two populations)
12mmX75mm tubes (up to four populations)
50ml tube (up to two populations in combination with 1.5ml tube)
1.5ml microcentrifuge tubes (up to four populations)
24,96,384 well plates
Nozzle/pressure: 100um 24psi (fixed)
*class II biosafety cabinet
Sony Synergy HAPS1 & 2 Cell Sorter*
Optics/Configurations: 4-laser, 14-color
Laser: 405
Filter 460/50BP
Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 520/35 BP
Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510
Laser: 488
Filter 530/40 BP
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 670/30 BP
PerCP, PI, 7AAD
Filter 695/40 BP
PerCP-Cy5-5
Laser: 561
Filter 585/29 BP
PE
Filter 610/20 BP
PE-Texas Red, Alexa Fluor610, mCherry, mKate, tdTomato
Filter 670/30 BP
PE-Cy5
Filter 750LP
PE-Cy7
Laser: 640
Filter 670/30 BP
APC, Alexa Fluor647, Sytox Red
Filter 720/40 BP
Alexa Fluor700
Filter 750 LP
APC-CY7, APC-H7
Software Version: Winlist 7.1
Sample Collection:
15ml tubes (up to two populations)
12mmX75mm tubes (up to four populations)
1.5ml microcentrifuge tubes (up to four populations)
24,96,384 well plates
Nozzle/pressure: 100um 24 psi (default), 70um 52psi (by request; call flow staff for more information)
*class II biosafety cabinet
LSRFortessa Cell Analyzer
Optics/Configurations: 4-laser, 13-color
Laser: 405
Filter 450/50BP
Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 525/50 BP
Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510
Filter 605/12 BP
Qdot605, Pacific Orange, BV605
Laser: 488
Filter 530/30 BP
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 710/50 BP
PerCP, PerCP-Cy5, PerCP-Cy5-5, PI, 7AAD
Laser: 561
Filter 585/15 BP
PE, DS Red
Filter 610/20 BP
PE-Texas Red, Alexa Fluor610, mCherry, mKate, tdTomato
Filter 660/20 BP
PE-Cy5
Filter 710/50
PE-Cy5.5
Filter 780/60 P
PE-Cy7
Laser: 640
Filter 670/14 BP
APC, Alexa Fluor647, Sytox Red
Filter 730/45 BP
Alexa Fluor700
Filter 780/60 BP
APC-CY7, APC-H7
Software Version: BD FACSDiva 6.1.3
Sample Acquisition: 12mmX75mm polystyrene tubes
Canto II Cell Analyzer
Optics/Configurations: 3-laser, 8-color
Laser: 405
Filter 450/50BP
Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 510/50 BP
Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510
Laser: 488
Filter 530/30 BP
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 585/42 BP
PE
Filter 670 LP
PE-Cy5, PerCP, PerCP-Cy5-5, PI, 7AAD
Filter 780/60 BP
PE-Cy7
Laser: 633
Filter 660/20 BP
APC, Alexa Fluor647, Sytox Red
Filter 685LP
Alexa Fluor700
Filter 780/60 BP
APC-CY7, APC-H7
Software Version: BD FACSDiva 6.1.3
Sample Acquisition: 12mmX75mm polystyrene tubes
HTS plate reader (96 well plates)
Calibur Cell Analyzer
Optics/Configurations: 2-laser, 4-color
Laser: 488
Filter 515-545
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 564-606
PE, DS Red
Filter 670-820
PerCP, PerCP-Cy5, PerCP-Cy5-5, PI, 7AAD
Laser: 635
Filter 653-669
APC, Alexa Fluor647, Sytox Red
Software Version: Cell Quest
Sample Acquisition: 12mmX75mm polystyrene tubes
Sony EC800Cell Analyzer
Optics/Configurations: 4-laser, 6-color
Co-linear cell analyzer: flexible color options
Software Version: EC800
Sample Acquisition: 12mmX75mm polystyrene tubes
96 well plate reader
Autosampler for 2x75 mm, PCR, or eppendorf tubes, and more than 10 plate formats
great for routine assays
BD Pathway 435 II Cell Imager
Optics/Configurations: 4-channel automated spinning disk
Excitation Filter 377/50 BP Emission Filter 435 LP
DAPI, Hoechst
Excitation Filter 482/35 BP Emission Filter 536/40
Alexa488, Calcein, Cell Tracker Green, Cy2, FITC, GFP,
Mito Tracker Green
Excitation Filter 543/22 BP Emission Filter 593/40
Alexa546, Alexa555, Alexa594, Cell Tracker Orange, Cell Tracker Red, Cy3, Mito Tracker Red, Propidium Iodide,
Texas Red, TRITC
Excitation Filter 543/22 BP Emission Filter 593/40
Alexa647, BODIPY, Cy5
Emission Filter 593/40
Transmitted Light
Software Version: BD Autovision 1.6
Sample Acquisition: 96,384 well plates (black photo bottom)
