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Sorting Instruments

FACSAria Fusion Cell Sorter*

Optics/Configurations: 6-laser, 15-color

Laser: 407

Filter 450/50 BP:
    Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 525/50 BP:
    Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510
Filter 610/20:
    BV605, Pacific Orange, Qdot 605
Filter 660/20:
    BV650, Qdot 655
Filter 710/50:
    BV711, Qdot 700
Filter 780/60:
    BV786, Qdot 800

Laser: 488

Filter 530/30 BP:
    Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 695/40 BP:
    PE-Cy5, PerCP, PerCP-Cy5, PerCP-Cy5.5, PI, 7AAD

Laser: 561

Filter 582/15:
    PE, DS Red
Filter 610/20:
    PE-Texas Red, Alexa Fluor610, mCherry, mKate, tdTomato
Filter 670/14:
    PE-Cy5
Filter780/60:
    PE-Cy7

Laser: 633

Filter 670/30 BP:
    APC, Alexa Fluor647, Sytox Red
Filter 730/45 BP:
    Alexa Fluor700
Filter 780/60:
    APC-Cy7, APC-H7

Lasers 375 and 445 additionally available. Contact flow staff for more information.

Software Version:
BD FACSDiva 8.0

Sample Tube: 12mm x 75mm polystyrene tube

Sample Collection:

15ml tubes (up to two populations)
12mm x 75mm tubes (up to four populations)
1.5ml microcentrifuge tubes (up to four populations)
24, 96, 384 well plates

Nozzle/Pressure: 70um/70psi (default), 100um/30psi (by request; call flow staff for more information)
*Class II Biosafety Cabinet

Influx Cell Sorter

Optics/Configurations: 5-laser, 14-color

Laser: 355 

Filter 460/50 BP:
    Hoechst Blue
Filter 670/30 BP:
    Hoechst Red

Laser: 405 

Filter 460/50 BP:
    Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 520/35 BP:
    Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510

Laser: 488 

Filter 530/40 BP:
    Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 670/30 BP:
    PerCP, PI, 7AAD
Filter 710/50 BP:
    PerCP-Cy5, PerCP-Cy5.5

Laser: 561 

Filter 585/29 BP:
    PE, DS Red
Filter 610/20 BP:
    PE-Texas Red, Alexa Fluor610, mCherry, mKate, tdTomato
Filter 670/30 BP:
    PE-Cy5
Filter 750 LP:
    PE-Cy7

Laser: 642 

Filter 670/30 BP:
    APC, Alexa Fluor647, Sytox Red
Filter 720/40 BP:  
    Alexa Fluor700
Filter 750 LP:
    APC-Cy7, APC-H7

Software Version: BD Sortware 1.0.0.650

Sample Tube: 12mm x 75mm polypropylene tube

Sample Collection:

15ml tubes (up to two populations)
12mm x 75mm tubes (up to four populations)
50ml tube (up to two populations in combination with 1.5ml tube)
1.5ml microcentrifuge tubes (up to four populations)
24, 96, 384 well plates

Nozzle/Pressure: 100um/24 psi (default), 70um/52psi (by request; call flow staff for more information)

FACSAria #1 Cell Sorter

Optics/Configurations: 3-laser, 9-color

Laser: 405

Filter 450/50 BP:
    Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 530/30 BP:
    Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510

Laser: 488 

Filter 530/30 BP:
    Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 576/26 BP:
    PE, DS Red
Filter 610/20 BP:
   PE-Texas Red
Filter 695/40 BP:
    PE-Cy5, PerCP, PerCP-Cy5, PerCP-Cy5.5, PI, 7AAD
Filter 780/60 BP:
    PE-Cy7

Laser: 633 

Filter 660/20 BP:
    APC, Alexa Fluor647, Sytox Red
Filter 780/60 BP:
    Alexa Fluor700, APC-Cy7, APC-H7

Software Version: BD FACSDiva 6.1.3

Sample Tube: 12mm x 75mm polystyrene tube

Sample Collection:

15ml tubes (up to two populations)
12mm x 75mm tubes (up to four populations)
1.5ml microcentrifuge tubes (up to four populations)
24, 96, 384 well plates

Nozzle/Pressure: 70um/70psi (default), 100um/30psi (by request; call flow staff for more information)

FACSAria #2 Cell Sorter

Optics/Configurations: 2-laser, 7-color

Laser: 488 

Filter 530/30 BP:
    Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 576/26 BP:
    PE, DS Red
Filter 610/20 BP:
    PE-Texas Red
Filter 695/40 BP:
    PE-Cy5, PerCP, PerCP-Cy5, PerCP-Cy5.5, PI, 7AAD
Filter 780/60 BP:
    PE-Cy7

Laser: 640 

Filter 660/20 BP:
    APC, Alexa Fluor647, Sytox Red
Filter 780/60 BP:
    Alexa Fluor700, APC-Cy7, APC-H7

Software Version: BD FACSDiva 6.1.3

Sample Tube: 12mm x 75mm polystyrene tube

Sample Collection:

15ml tubes (up to two populations)
12mm x 75mm tubes (up to four populations)
1.5ml microcentrifuge tubes (up to four populations)

Nozzle/Pressure: 70um/70psi (default), 100um/30psi (by request; call flow staff for more information)

FACSJazz Cell Sorter*

Optics/Configurations: 2-laser, 6-color

Laser: 488 

Filter 530/40 BP:
    Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 576/26 BP:
    PE, DS Red
Filter 629/40 BP:
    PerCP, PerCP-Cy5, PerCP-Cy5-5, PI, 7AAD
Filter 750 LP:
    PE-Cy7

Laser: 640 

Filter 670/30 BP:
    APC, Alexa Fluor647, Sytox Red
Filter 750 LP:
    APC-Cy7, APC-H7

Software Version: BD Sortware 1.0.0.650

Sample Tube: 5ml polypropylene tube

Sample Collection:

15ml tubes (up to two populations)
12mm x 75mm tubes (up to four populations)
50ml tube (up to two populations in combination with 1.5ml tube)
1.5ml microcentrifuge tubes (up to four populations)
24, 96, 384 well plates

Nozzle/Pressure: 100um/24psi (fixed)
*class II biosafety cabinet


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