Equipment

North Campus

Cell Sorters

Becton Dickinson FACSAria (three-laser, 11-parameter) high speed, digital cell sorter.

The Aria is a state-of-the-art cell sorter with the ability to analyze nine fluorochromes and sort four subpopulations using the new optical bench and fiber-optic light path collection.

Becton Dickinson FACSVantage SE Turbo cell sorter with UV and blue laser line

Cytometers

Becton Dickinson LSR II analytical flow cytometer (four-laser, 12-parameter) with 96-well plate sampler

Becton Dickinson FACSArray flow cytometer (two-laser, four-color) with 96-well plate auto-sampler for high-throughput assays

Two Becton Dickinson FACSCalibur flow cytometers (two-laser, four-color)

Compucyte LSC

Slide-based system with fluorescence detection and quantitation can analyze up to three fluorochromes from cells deposited on slides. Protein expression data can be obtained from as few as several hundred cells.

This set-up includes two new cytometry data analysis workstations (Mac and PC) for offline analysis with different software packages including FlowJo (Treestar, Inc.), Modfit (Verity Software House), FCS Express (DeNovo), WinMDI (Scripss Institute), CellQuestPro and DIVA (both by Becton Dickinson), allowing detailed analysis of multiparameter data, export of statistics to spreadsheets and production of publication quality figures.

Microscopes

Olympus FV500 laser scanning confocal microscope (four-laser, three-color with UV excitation laser

Set-up includes the Olympus microscope-based DIMscan system for high-throughput fluorescence-based viable cell assays in a 96-well plate format.

Olympus DSU Spinning Disc Confocal Microscope System

This system is for live cell imaging with real-time camera detection in addition to a computer controlled stage and incubator. It has been newly upgraded with a faster, more sensitive Orca II ER Camera and a Wafergen Smartslide Micro-Incubation System.