Equipment
FACS Calibur (Becton Dickinson) Flow Cytometer
This instrument has the ability to analyze up to 10 fluorochromes simultaneously.
LSR-II (Becton Dickinson) Flow Cytometer
This instrument is equipped with four-laser excitation and can analyze up to eight fluorochromes from one cell.
Vantage Turbo-Sort-SE (Becton Dickinson) Cell Sorter
This instrument has enhanced capabilities including a 10-fold increase in sort rate and the use of polygonal sort regions. The instrument utilizes a 488/UV system.
Laser Scanning Cytometer (CompuCyte)
This instrument allows quantitation of three-color fluorescence-tagged intracellular proteins on as few as 100-200 cells deposited (or FACS sorted) on slides.
FV500 Fluoview Confocal Laser Scanning Microscope (Olympus)
The FV500 Fluoview is a versatile, multi-user instrument with four simultaneous primary image ports (up to nine total imaging ports), high-resolution imaging and analysis software with 3D visualization, and deconvolution software. The instrument has four laser excitation wavelengths: 488 nm argon, 543.5 nm green helium neon, 633 nm red helium neon and 405 nm violet. It is equipped with 10X, 20X, 40X, 60X objectives (total of eight), temperature-controlled microscope stage and a micro-environmental chamber (Delta T4) for confocal microscopy of live cells. Up to four images/second can be obtained. Data output is on CD, CD-ROM, ZIP or paper hardcopy. The instrument will be networked to allow users off-line analysis.
LSM 510 Confocal Laser Scanning Microscope (Zeiss)
This instrument contains three laser sources (argon 488/514 nm, helium/neon 543 nm, and helium/neon 633 nm); a Zeiss axiovert 100/135 baseport microscope with a motorized scanning stage, x, y, z manual and computer-driven control, transmitted (12V/100W halogen) and fluorescent light (HBO 50W) sources; appropriate objectives (10X, 25X, 40X and 63X; the 25X objective being a multiple immersion lens to allow work with tissues mounted in aqueous solutions); a transmitted-light photo-multiplier tube; the LSM 510 3-channel scanning control module; and the LSM 510 control computer and software. System upgrades now allow us to scan polygonal regions of interest as well as to utilize different excitation configurations and obtain specific emission acquisitions on sequential passes of the object, thus obviating emission spectral overlap.
The instrument allows simultaneous analysis of four detection channels (three fluorescence and one transmitted). All questions regarding this instrument should be referred to Dr. Nicholas Terry at 713-792-3424.
New Equipment
FACSAria Cell Sorter (Becton Dickinson)
The Aria is the latest generation of high-performance, high-speed and four-way sorting flow cytometry instrumentation. It incorporates fixed alignment cuvette sorting technology, which increases sensitivity on a flexible choice of fluorochromes with up to 15 signals available at one time.
Olympus IX81 Fluorescent Imaging System (Olympus)
The Olympus IX81 fluorescent imaging system offers unparalleled flexibility in fixed and live-cell microscopy. It offers four-port access to allow the additional detector capabilities. The Olympus IX81 will also be fitted with the latest patented 100X 1.45NA objective, which will offer increased resolution and allow more discreet analysis of sub-cellular function.
The BD FACSArray Bioanalyzer (Becton Dickinson)
The BD FACSArray bioanalyzer is designed for multiparameter analysis of proteins in immunology, cell and cancer biology applications. It is a dual laser system with the capability of analyzing up to 15,000 events per second with acquisition of a 96-well plate in less than 35 minutes.