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Analysis Instruments

LSRFortessa X-20 Cell Analyzer

Optics/Configurations: 5-laser, 18-color

Laser: 355

Filter 379/28 BP:
    Hoechst Blue, BUV396
Filter 740/35 BP:
    BUV737

Laser: 405

Filter 450/50 BP:
    Alexa Fluor 405, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Violet, BV421, DAPI
Filter 525/50 BP:
    Alexa Fluor 430, AmCyan, CFP, Live/Dead fixable Aqua, BV510
Filter 610/20 BP:
    BV605, Pacific Orange, Qdot 605
Filter 670/30 BP:
    BV650, Qdot 655
Filter 710/50 BP:
    BV711, Qdot 700
Filter 780/60 BP:
    BV786, Qdot 800

Laser: 488

Filter 530/30 BP:
    Alexa Fluor 488, FITC, GFP, Sytox Green, Calcien Green
Filter 710/50 BP:
    PerCP, PerCP-Cy5, PerCP-Cy5.5, 7-AAD

Laser: 561

Filter 586/15 BP:
    PE, DS Red, PI
Filter 610/20 BP:
    PE-Texas Red, PE-Alexa 610, PE-CF594, Live/Dead Fixable Red, Alexa Fluor 610, mCherry, mKate, tdTomato
Filter 670/30 BP:
    PE-Cy5
Filter 710/50 BP:
    PE-Cy5.5, PE-Alexa 700
Filter 780/60 BP:
    PE-Cy7

Laser: 640

Filter 670/30 BP:
    APC, Alexa Fluor 647, Alexa Fluor 680, Sytox Red
Filter 730/45 BP:
    Alexa Fluor 700, APC-Alexa 700, APC-R700
Filter 780/60 BP:
    APC-Cy7, APC-H7

Software Version: BD FACSDiva 8.0

Sample Acquisition:

12mm x 75mm polystyrene tubes
HTS plate reader (96, 384 well plates)

LSRFortessa Cell Analyzer

Optics/Configurations: 5-laser, 16-color

Laser: 355

Filter 450/50BP:
    DAPI, Hoechst Blue, BUV396
Filter 730/45BP:
    BUV737

Laser: 405

Filter 450/50 BP:
    Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 525/50 BP:
    Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510
Filter 605/12 BP:
    Qdot605, Pacific Orange, BV605
Filter 670/30BP:
    BV650, Qdot 655

Laser: 488 

Filter 530/30 BP:
    Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 710/50 BP:
    PerCP, PerCP-Cy5, PerCP-Cy5.5, 7AAD

Laser: 561 

Filter 585/15 BP:
    PE, DS Red, PI, Alexa Fluor555
Filter 610/20 BP:
    PE-Texas Red, Alexa Fluor610, mCherry, mKate, tdTomato, Alexa Fluor594
Filter 660/20 BP:
    PE-Cy5
Filter 710/50 BP:
    PE-Cy5.5
Filter 780/60 BP:
    PE-Cy7

Laser: 640 

Filter 670/14 BP:
    APC, Alexa Fluor647, Sytox Red
Filter 730/45 BP:
    Alexa Fluor700
Filter 780/60 BP:
    APC-Cy7, APC-H7

Software Version: BD FACSDiva 8.0

Sample Acquisition:

12mm x 75mm polystyrene tubes
HTS Plate Reader (96, 384 well plates)

FACSCanto II Cell Analyzer

Optics/Configurations: 3-laser, 8-color

Laser: 405 

Filter 450/50 BP:
    Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 510/50 BP:
    Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510

Laser: 488 

Filter 530/30 BP:
    Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 585/42 BP:
    PE
Filter 670 LP:
    PE-Cy5, PerCP, PerCP-Cy5.5, PI, 7AAD
Filter 780/60 BP:
    PE-Cy7

Laser: 633 

Filter 660/20 BP:
    APC, Alexa Fluor647, Sytox Red
Filter 685 LP:
    Alexa Fluor700
Filter 780/60 BP:
    APC-Cy7, APC-H7

Software Version: BD FACSDiva 8.0

Sample Acquisition:

12mm x 75mm polystyrene tubes
HTS plate reader (96 well plates)

Gallios Cell Analyzer

Optics/Configurations: 3-laser, 10-color

Laser: 405

Filter 450/40 BP:
    Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 550/40 BP:
    Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510, Pacific Orange

Laser: 488

Filter 525/40 BP:
    Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 575/30 BP:
    PE, DS Red
Filter 620/30 BP:
    PE-Texas Red, PE-Cy5
Filter 695/30 BP:
    PE-Cy5.5, PerCP, PerCP-Cy5.5, PI, 7AAD
Filter 755 LP:
    PE-Cy7

Laser: 638

Filter 660/20 BP:
    APC, Alexa Fluor647, Sytox Red
Filter 725/20 BP:
    Alexa Fluor700
Filter 755 LP:
    APC-Cy7, APC-H7

Software Version: Gallios 1.2

Sample Tube:

12mm x 75mm polystyrene tubes
32 tube Multi Carousel Loader option

FACSCalibur Cell Analyzer

Optics/Configurations: 2-laser, 4-color

Laser: 488 

Filter 515-545:
    Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 564-606:
    PE, DS Red
Filter 670-820:
    PerCP, PerCP-Cy5, PerCP-Cy5.5, PI, 7AAD

Laser: 635

Filter 653-669:
    APC, Alexa Fluor647, Sytox Red

Software Version: Cell Quest Pro 0.3.ff1b

Sample Acquisition:

12mm x 75mm polystyrene tubes

Eclipse EC800 Cell Analyzer

Optics/Configurations: 4-laser, 6-color
Co-linear cell analyzer: flexible color options

Software Version: EC800 1.3.4

Sample Acquisition:

12mm x 75mm polystyrene tubes
96 well plate reader
Autosampler for 12mm x 75 mm, PCR, or eppendorf tubes, and more than 10 plate formats


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