Skip to Content

Microarray Services – Overview

The SMF’s microarray service offers a comprehensive set of integrated gene expression services that facilitate studying the role of genomic alterations and transcriptional regulation of genes.

The SMF provides complete microarray services including:

  • RNA and DNA sample quality check
  • Target preparation
  • Hybridization Services
  • Imaging Services
  • Signal quantification

Supported Applications:

  • Gene Expression profiling
  • MicroRNA analysis
  • Single Nucleotide Polymorphism (SNP) genotyping
  • Methylation Analysis

These applications can be utilized to detect and map chromosomal aberrations and SNPs, profile gene expression patterns, identify differences between two populations of DNA or RNA (i.e., tumor vs. normal cell populations), determine the effect of therapeutic agents on the genome and their effect on gene expression, and determine the effect of genetic alterations on gene expression patterns

The table below summarizes the SMF’s microarray applications, platforms and supported arrays. Click on the link for information on each array type.

 

Array Type

Sample Requirements

Minimum Submission

Gene Expression 

 Affymetrix Express 3'IVT,T

≥100 ng total RNA (minimum 33ng/uL), 12 uL/sample.

1 sample

 Affymetrix Human  Gene 1.0 ST Array

≥100 ng total RNA (minimum 33ng/uL),12 uL/sample.  One sample per array cartridge.

1 sample

 Affymetrix Human Exon 1.0 S Array

≥100 ng total RNA (minimum 33ng/uL) 12 uL/sample.  One sample per array cartridge.

1 sample

Illumina HumanHT12v4 (24 rxn)

≥100-200 ng total RNA (minimum 33 ng/uL),12 uL/sample.

12 samples

Illumina MouseWG-6 v2 (12 rxn)

≥100-200 ng total RNA (minimum 33 ng/uL),12 uL/sample.

6  samples

Illumina MouseRef-8 v2 (16 rxn)

≥100-200 ng total RNA (minimum 33 ng/uL), 12 uL/sample.

16 samples

Illumina Gene Expression-FFPE

≥100-200 ng total RNA (minimum 33 ng/uL).   12 uL/sample. 

24 samples

SNP Profiling

Affymetrix SNP 6.0 Genotyping

≥500 ng of genomic DNA (minimum 50 ng/uL),    15 uL/sample

30 samples  Note:  One sample per array cartridge

Affymetrix CytoScan HD CNV

≥250 ng of genomic DNA (minimum 50 ng/uL), 12 uL/sample

1 sample

Illumina HumanCytoSNP-12

≥200 ng of genomic DNA (minimum 50 ng/uL), 12 uL/sample

12 samples

Illumina HumanOmniExpress-12

≥200 ng of genomic DNA (minimum 50 ng/uL).,    12 uL/sample

48 samples

Illumina HumanOmni1S-8

≥200 ng of genomic DNA (minimum 50 ng/uL).   12 uL/sample

16 samples

Illumina HumanOmni2.5-8

≥200 ng of genomic DNA (minimum 50 ng/uL), 12 uL/sample

16 samples

Illumina HumanOmni2.5S-8

≥200 ng of genomic DNA (minimum 50 ng/uL),    12 uL/sample

16 samples

Illumina HumanOmni5-4

≥400 ng of genomic DNA (minimum 50 ng/uL),    16 uL/sample

16 samples

Illumina HumaOminExpress-FFPE-12

≥200 ng of genomic DNA (minimum 50 ng/uL), 12 uL/sample.

24 samples

Illumina BeadXpress Custom arrays

≥200 ng of genomic DNA (minimum 50 ng/uL), 12 uL/sample.

48 samples

Methylation

Illumina HumanMethylation450 Infinium

≥500 ng of genomic DNA (minimum 50 ng/uL), 12 uL/sample.

24 samples

Illumina FFPE HumanMethylation

≥250 ng of genomic DNA (minimum 50 ng/uL),  12 uL/sample.

24 samples


© 2014 The University of Texas MD Anderson Cancer Center