Microarray Services – Overview
The SMF’s microarray service offers a comprehensive set of integrated gene expression services that facilitate studying the role of genomic alterations and transcriptional regulation of genes.
The SMF provides complete microarray services including:
- RNA and DNA sample quality check
- Target preparation
- Hybridization Services
- Imaging Services
- Signal quantification
Supported Applications:
- Gene Expression profiling
- MicroRNA analysis
- Single Nucleotide Polymorphism (SNP) genotyping
- Methylation Analysis
These applications can be utilized to detect and map chromosomal aberrations and SNPs, profile gene expression patterns, identify differences between two populations of DNA or RNA (i.e., tumor vs. normal cell populations), determine the effect of therapeutic agents on the genome and their effect on gene expression, and determine the effect of genetic alterations on gene expression patterns
The table below summarizes the SMF’s microarray applications, platforms and supported arrays. Click on the link for information on each array type.
Array Type | Sample Requirements | Minimum Submission |
|---|---|---|
Affymetrix Express 3'IVT,T | ≥100 ng total RNA (minimum 33ng/uL), 12 uL/sample. | 1 sample |
Affymetrix Human Gene 1.0 ST Array | ≥100 ng total RNA (minimum 33ng/uL),12 uL/sample. One sample per array cartridge. | 1 sample |
Affymetrix Human Exon 1.0 S Array | ≥100 ng total RNA (minimum 33ng/uL) 12 uL/sample. One sample per array cartridge. | 1 sample |
Illumina HumanHT12v4 (24 rxn) | ≥100-200 ng total RNA (minimum 33 ng/uL),12 uL/sample. | 12 samples |
Illumina MouseWG-6 v2 (12 rxn) | ≥100-200 ng total RNA (minimum 33 ng/uL),12 uL/sample. | 6 samples |
Illumina MouseRef-8 v2 (16 rxn) | ≥100-200 ng total RNA (minimum 33 ng/uL), 12 uL/sample. | 16 samples |
Illumina Gene Expression-FFPE | ≥100-200 ng total RNA (minimum 33 ng/uL). 12 uL/sample. | 24 samples |
Affymetrix SNP 6.0 Genotyping | ≥500 ng of genomic DNA (minimum 50 ng/uL), 15 uL/sample | 30 samples Note: One sample per array cartridge |
Affymetrix CytoScan HD CNV | ≥250 ng of genomic DNA (minimum 50 ng/uL), 12 uL/sample | 1 sample |
Illumina HumanCytoSNP-12 | ≥200 ng of genomic DNA (minimum 50 ng/uL), 12 uL/sample | 12 samples |
Illumina HumanOmniExpress-12 | ≥200 ng of genomic DNA (minimum 50 ng/uL)., 12 uL/sample | 48 samples |
Illumina HumanOmni1S-8 | ≥200 ng of genomic DNA (minimum 50 ng/uL). 12 uL/sample | 16 samples |
Illumina HumanOmni2.5-8 | ≥200 ng of genomic DNA (minimum 50 ng/uL), 12 uL/sample | 16 samples |
Illumina HumanOmni2.5S-8 | ≥200 ng of genomic DNA (minimum 50 ng/uL), 12 uL/sample | 16 samples |
Illumina HumanOmni5-4 | ≥400 ng of genomic DNA (minimum 50 ng/uL), 16 uL/sample | 16 samples |
Illumina HumaOminExpress-FFPE-12 | ≥200 ng of genomic DNA (minimum 50 ng/uL), 12 uL/sample. | 24 samples |
Illumina BeadXpress Custom arrays | ≥200 ng of genomic DNA (minimum 50 ng/uL), 12 uL/sample. | 48 samples |
Illumina HumanMethylation450 Infinium | ≥500 ng of genomic DNA (minimum 50 ng/uL), 12 uL/sample. | 24 samples |
Illumina FFPE HumanMethylation | ≥250 ng of genomic DNA (minimum 50 ng/uL), 12 uL/sample. | 24 samples |