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Getting Started

Submit for STR
Submit for Mutation Detection (Sequenom)
Submit for Mycoplasma
Request/Deposit Cell Lines

For All Services

  •   HOW DO I SUBMIT SAMPLES OR REQUEST CELL LINES?

***Please select one of the following criteria below and complete the Submission Form AND Sample List (PCR Plate Template is for Sequenom submissions only)***

WE WILL NO LONGER ACCEPT SAMPLES WITHOUT BOTH THE UPDATED SUBMISSION FORM AND SAMPLE LIST FILLED OUT!

MD Anderson Labs Submission/Request Forms

External Academic Labs Submission/Request Forms

Non-Academic and International Labs Submission/Request Forms

 Please email CCSGCCLC@mdanderson.org to set an appointment time for sample submission

  • WHO DO I CONTACT FOR SAMPLE SUBMISSION?
    Keri Sherman, CCSGCCLC@mdanderson.org
    Research Data Specialist
    2SCR2.2207
    The University of Texas MD Anderson Cancer Center
    South Campus Research Building II
    7435 Fannin Street
    Houston, TX 77054
    713-792-5743

**YOU WILL NEED TO SET AN APPOINTMENT FOR DROP OFF. WE CANNOT GUARANTEE ACCEPTANCE OF YOUR SAMPLES IF YOU DO NOT MAKE AN APPOININTMENT!**

Submit for STR

ONLY HUMAN DNA, NOT MOUSE OR ANY OTHER ANIMAL

**Please note that unless the whole plate for STR Fingerprinting fails for some reason, we will not re-run failed samples free of charge**

  • Can I send DNA or cells?
    We only accept DNA.
  • How do I extract DNA?
    You can use any column purification method. We suggest Qiagen (cat. 51306), see Protocol or you can contact the Biospecimen Extraction Facility.
  • What concentration do you need?
    We need the sample dilution to be at 10 ng/uL.
    • Make sure when diluting DNA that you use Amplification Water or PCR Certified Water
  • What volume do you need?
    We need 50uL.
  • How long is the turn-around?
    1 week. We do one run per week on Monday. Samples must be submitted by the previous Friday before 3:30pm to ensure they get put on the plate for Monday.

Submit for Mutation Detection (Sequenom)

If you have more than 24 samples please put them on a 96-well PCR plate. We can supply the plate that works with our robot if necessary.

  • Can I send DNA or cells?
    We only accept DNA.
  • Will you return unused sample?
    Yes. We only keep unused sample for 6 months.
  • Can I use FFPE samples?
    FFPE extracted DNA can be used for Sequenom analysis, but the quality of the DNA may affect the results.
  • How do I extract DNA?
    You can use any column purification method. We suggest Qiagen (cat. 51306). The DNA must have an A260/A280 ratio of at least 1.7. See Protocol, or you can contact the Biospecimen Extraction Facility.
  • What concentration do you need?
    We need the sample dilution to be at 10ng/uL.
  • What volume to you need?
    We need 100uL.
  • How long is the turn-around?
    1-2 months, depending on work flow.

We have 2 separate panels we can run for mutation detection. Click to see the lists for Panels A and B.

    *Note that if you would like us to run both panels the charge will be doubled, otherwise you can pick either Panel A or Panel B. Please see Submission Form for pricing. 

Submit for Mycoplasma

  • What kit do you use?
    Lonza, MycoAlert kit
  • What do you need?
    We need 1-2mL of media that the cells were grown in. NOTE: Please spin out any suspended cells within the media.
  • How do I store my media samples?
    Store at 4oC for 1 week, or 20oC for more than 1 week
  • How long is the turn-around?
    1 week, depending on work flow.
  • HOW MUCH IS THE SERVICE?
    Please see Services page.

Payment Methods:

INSIDE MDACC: PeopleSoft Account Number
OUTSIDE MDACC: Our preferred method of payment is by Credit Card. You can complete the Credit Card Authorization Form and submit with your Submission Forms. We can also accept a PO (Purchase Order) or Wire Transfer if paying by credit card is not possible.

 

Request/Deposit Cell Lines

Request

You will need to fill out our Submission/Request Form to request a cell line. Those can be found of the top of this page. Please download the appropriate PDF form (Internal Academic, External Academic, or Non-Academic/International). You will also need to complete the Credit Card Authorization Form for Payment, or will need to get a PO (Purchase Order) from your institution. 

Here is the List of Cell Lines that we can currently distribute freely. For all other inquires, please contact the Cell Line Core.

  • How long does it take to get cells?
    • Internal Customers (Inside MD Anderson)
      • Since we are trying to revalidate and test all our cell lines before distribution the turnaround time is approximately 2 weeks (Depending on the growth of the cells). If we have already tested that particular cell line, then the turnaround time is 2-3 days.
    • External customers
      • An MTA will be needed. This process can take between 1-3 months. Please fill out the appropriate Submission Form from the top of the page and email to CCSGCCLC@mdanderson.org. Once we receive your completed form by email, this will serve as your request to purchase a cell line and the CCLC will start the MTA on our end.

Deposit

For any PI who is willing to allow cell lines to be shared with other internal or external users of the MD Anderson Characterized Cell Line Core (CCLC)--(this excludes cell lines obtained from ATCC OR cell lines containing any restrictions under an MTA). We will store the cells and provide a vial of that specific cell line at no cost when requested by the depositing PI. This provides our Core the opportunity to expand the number of cell lines within the Core. This also benefits the PI by providing them with a specific site to store their modified cell lines and eliminating the cost for expanding cell lines for other requestors, since the Core already provides this service. This storage is guaranteed for a period of up to three (3) years from deposit date.  This timeline is necessary to help maintain our quality control since we cannot guarantee that the vial will still contain viable cells after a period of time.

Please fill out the Cell Line Deposit Form and submit it to CCSGCCLC@mdanderson.org. You will also need to submit written permission that the Cell Line Core has permission to freely distribute your cell line.


© 2014 The University of Texas MD Anderson Cancer Center