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Getting Started

Preparing for STR
Preparing for Mutation Detection (Sequenom)
Preparing for Mycoplasma Testing
Request/Deposit Cell Lines

For All Services

  •   HOW DO I SUBMIT SAMPLES OR REQUEST CELL LINES?

Go to mdanderson.ilabsolutions.com to create your account and begin initiating your CCLC Service Requests

Once your online request has been initiated and approved, you will need to e-mail CCSGCCLC@mdanderson.org to set an appointment time for sample submission

Please print off your online request form and submit with your samples

  • WHO DO I CONTACT FOR SAMPLE SUBMISSION?
    Keri Sherman
    Research Data Specialist
    2SCR2.2207
    MD Anderson Cancer Center
    7435 Fannin Street
    Houston, TX 77054
    713-792-5743

**YOU WILL NEED TO SET AN APPOINTMENT FOR SAMPLE DROP OFF. WE CANNOT GUARANTEE ACCEPTANCE OF YOUR SAMPLES IF YOU DO NOT MAKE AN APPOININTMENT!** APPOINTMENT TIMES CAN BE MADE, MONDAY-FRIDAY BETWEEN THE HOURS OF 9:00AM-4:00PM.

Preparing for STR

ONLY HUMAN DNA, NOT MOUSE OR ANY OTHER ANIMAL

**Please note that unless the whole plate for STR Fingerprinting fails for some reason, we will not re-run failed samples free of charge**

  • Can I send DNA or cells?
    We only accept DNA.
  • How do I extract DNA?
    You can use any column purification method. We suggest Qiagen (cat. 51306), see Protocol or you can contact the Biospecimen Extraction Facility.
  • What concentration do you need?
    We need the sample dilution to be at 10 ng/uL.
    • Make sure when diluting DNA that you use Amplification Water or PCR Certified Water
  • What volume do you need?
    We need 50uL.
  • How long is the turn-around time?
    1 week. We do one run per week on Monday. Samples must be submitted by the previous Friday before 4:00 pm to ensure they get put on the plate for Monday.
  • What are the prices for STR?
    Inside MDACC the charge is $50 per sample. Outside MDACC the charge is $70 per sample + 60% overhead charge (Any UT Health Science Center and Baylor are exempt from paying the 60% overhead charge).

Preparing for Mutation Detection (Sequenom)

If you have more than 24 samples please put them on a 96-well PCR plate. We can supply the plate that works with our robot if necessary.

  • Can I send DNA or cells?
    We only accept DNA.
  • Will you return unused sample?
    Only if this is requested upfront. We only keep unused samples for 6 months.
  • Can I use FFPE samples?
    FFPE extracted DNA can be used for Sequenom analysis, but the quality of the DNA may affect the results.
  • How do I extract DNA?
    You can use any column purification method. We suggest Qiagen (cat. 51306). The DNA must have an A260/A280 ratio of at least 1.7. See Protocol, or you can contact the Biospecimen Extraction Facility.
  • What concentration do you need?
    We need the sample dilution to be at 10ng/uL.
  • What volume to you need?
    We need 100uL.
  • How long is the turn-around time?
    1-2 months, depending on work flow.
  • What are the prices for Sequenom?
    Inside MDACC the charge is $100 per sample. Outside MDACC the charge is $140 per sample + 60% overhead charge (Any UT Health Science Center and Baylor are exempt from paying the 60% overhead charge).

  

We have 2 separate panels we can run for mutation detection. Click to see the lists for Panels A and B.

    *Note that if you would like us to run both panels the charge will be doubled, otherwise you can pick either Panel A or Panel B.  

Preparing for Mycoplasma Testing

  • What kit do you use?
    Lonza, MycoAlert kit
  • What do you need?
    We need 1mL of media that the cells were grown in. NOTE: Please spin out any suspended cells within the media.
  • How do I store my media samples?
    Store at 4oC for 1 week, or 20oC for more than 1 week
  • How long is the turn-around time?
    1 week, depending on work flow.
  • What are the prices for Mycoplasma Testing?
    Inside MDACC the charge is $20 per sample. Outside MDACC the charge is $30 per sample + 60% overhead charge (Any UT Health Science Center and Baylor are exempt from paying the 60% overhead charge).

Payment Methods:

INSIDE MDACC: PeopleSoft Account Number

OUTSIDE MDACC: Our preferred method of payment is by Credit Card. You can complete the Credit Card Authorization Form and upload it with your online request (The credit card form and instructions are also on the iLab site). We can also accept a PO (Purchase Order) or Wire Transfer if paying by credit card is not possible. Please Click Here to obtain MDACC Institutional Information to help you create your PO.

 

Request/Deposit Cell Lines

Request

You will need to fill out the online service request at mdanderson.ilabsolutions.com

Here is the List of Cell Lines that we can currently distribute. 

  • How long does it take to get cells?
    • Internal Customers (Inside MD Anderson)
      • Since we are trying to revalidate and test all our cell lines before distribution the turnaround time is approximately 2 weeks (Depending on the growth of the cells). If we have already tested that particular cell line, then the turnaround time is 2-3 days.
    • External customers
      • An MTA will be needed. This process can take between 1-3 months. Once your online request has been approved, we will start an MTA on our end, and once complete will ship the cell line out.
  • What are the prices for purchasing a cell line?
    Inside MDACC the charge is $100 per cell line (No shipping fees apply). Outside MDACC the charge is $140 per cell line + 60% overhead charge + $100 domestic shipping/handling fee OR $800 international shipping/handling fee (Any UT Health Science Center and Baylor are exempt from paying the 60% overhead charge).

Deposit

For any PI who is willing to allow cell lines to be shared with other internal or external users of the MD Anderson Characterized Cell Line Core (CCLC)--(this excludes cell lines obtained from ATCC OR cell lines containing any restrictions under an MTA). We will store the cells and provide a vial of that specific cell line at no cost when requested by the depositing PI. This provides our Core the opportunity to expand the number of cell lines within the Core. This also benefits the PI by providing them with a specific site to store their modified cell lines and eliminating the cost for expanding cell lines for other requestors, since the Core already provides this service. This storage is guaranteed for a period of up to three (3) years from deposit date.  This timeline is necessary to help maintain our quality control since we cannot guarantee that the vial will still contain viable cells after a period of time.

You can request the deposit of your cell lines through mdanderson.ilabsolutions.com


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