Services
DNA Extraction
DNA can be extracted from whole blood, fresh or frozen tissues, cultured cells and archival tissues. The methodology depends on the source of the tissue.
Microsatellite Instability Testing
Tumor tissue can be tested for microsatellite instability using DNA isolated from paired tumor tissue and blood from the same patient. The markers used are BAT25, BAT26, D2S123, D5S346 and D17S250. Additional markers can be tested upon request.
PCR and Fragment Analysis
DNA can be subjected to PCR and the PCR product is then analyzed by single strand conformational polymorphism or it can be separated by size using gel electrophoresis. This approach can be used for genotyping samples at polymorphic loci.
RNA Extraction from Fresh Frozen Tissue
The method used for this is dependent on the tissue type. Currently it is done manually, but we plan to develop strategies with our new Autogen Extraction Machine and the new ABI PRISM 6100 Nucleic Acid PrepStation.
Simultaneous Isolation of RNA, DNA and Protein
This is done using a QIAGEN kit and is most useful for non-laboratory based investigators.
cDNA Preparation and RT-PCR from RNA
RNA that has been extracted by the facility can then be used as a template for cDNA preparation and RT-PCR of target sequence. The product is of sufficient quality to submit to the DNA Analysis Facility.
Allele-Specific Discrimination
This service is performed using the ABI PRISM 7700 Sequence Detection System. It is a PCR-based service that detects SNPS that are commonly studied in genetic and epidemiological studies.
SNP Analysis by Pyrosequencing
This is a high-throughput SNP analysis by sequencing. The machine can analyze 98 PCR products in 15 to 30 minutes.
CpG Island Methylation Assay
This assay is also done on the pyrosequencer.
Price List
Services and Charges
- DNA extraction from peripheral blood leukocytes: $10
- MSI testing: $260/pair
- PCR and fragment analysis of DNA: $8/sample
- RNA, DNA and protein extraction: $100/sample*
- RNA extraction from solid tissue: $100/sample*
- cDNA preparation and RT-PCR from RNA: $50/sample
- DNA extraction from solid tissue: $20/sample
* Reduced prices available when submitting multiple samples.