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Equipment

AutogenGenFlex Star

The Flex STAR is an automated DNA Extraction Instrument, optimized for use with Qiagen chemistry. Combining AutoGen's robust and reliable instrumentation and Qiagen's proven FlexiGene chemistry, the STAR extracts DNA from 1.0 to 10.0 mls of whole blood.

PSQ HS 96 Pyrosequencer

PSQ HS 96 Pyrosequencer is used for the detection of mutations and SNPs in a variety of clinical applications, and the analysis of DNA methylation. PSQ HS 96 is a highly sensitive and quantitative genetic analysis system based on Pyrosequencing technology. Pyrosequencing stands out among rapid genetic analysis methods in that it always generates true sequence information. This gives per-sample quality control in every run, from which the software makes an automatic quality assessment. Results are therefore unambiguous and eliminate the need to repeat assays or run separate controls. Each result, called a Pyrogram™, is a quantitative representation of the sample's nucleotide base sequence. Every Pyrogram shows determined polymorphic position and is ideal for quantitative analysis.

Molecular Devices SpectraMax PLUS384

MD Anderson recently purchased a Molecular Devices SpectraMax PLUS384 high-throughput microplate spectrophotometer for the BER. This instrument can measure absorbances for a single cuvette as well as in the 96-well and 384-well format. We are currently using it for quantitating protein yields.

Molecular Devices Spectramax Gemini EM Fluorescence Microplate Reader

This machine is a high throughput reader (up to 384 well plates) capable of detecting fluorescence assays. Dual monochromators are able to detect variable wavelengths between 250-8 50nm. Up to 4 wavelength pairs can be assessed for endpoint and kinetic readings. This plate reader is used for assays including Picogreen for double-stranded DNA quantitation.

Thermo Scientific Legend Micro 21R Sorvall Refrigerated Centrifuge

This centrifuge was purchased for the BER by MD Anderson for manual RNA extraction. The Sorvall is able to effectively separate out RNA due to its low temperature/high speed capabilities. 

PTC-200 DNA Engine, MJ Research, Inc.

This is a thermal cycler used for generating PCR products. It has interchangeable 48-well and 96-well blocks for carrying out PCRs.

Tecan Infinite M200 NanoQuant Microplate Reader 

The NanoQuant is a spectrophotometer used for general quantitation of RNA and DNA. This device is capable of quantitating 16 samples at a time. Additionally, 2uL input is all that is necessary for accurate measurement of high quality samples.


© 2014 The University of Texas MD Anderson Cancer Center