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Services

The Center for RNA Interference and Non-Coding RNAs (RNA Center) is now providing high-throughput in situ hybridization (ISH) technology for microRNA and non-coding RNA analysis using Ventana Discovery Ultra. The Core provides equipment and services to determine gene expression patterns in mouse and human tissues.

Equipment:Ventana

  • VENTANA DISCOVERY ULTRA

Flexibility:

  • Unmatched protocol flexibility for rapid assay development &
  • Run 30 completely independent protocols for improved workflow

Reproducibility:

  • Fully automated slide processing, baking through staining
  • Ready-to-use optimized reagents
  • Apply precise heating to each slide

Materials and Methods:

  • Frozen and FFPE tissue samples
  • microRNA probe or custom LNA probe from Exiqon
  • other ncRNA specific probes with LNA technology
  • Detection with anti-digoxigenin or anti-FITC antibody /AP conjugated 2nd antibody/ NBT-BCIP substrate
  • Imaging in a bright-field microscope
  • Data interpretations are at the discretion of the investigator (we will provide a quantification of the intensity of the signal by using Image-Pro Plus Software)

Processing Time:

  • Up to 7 business days for miRNA hybridization
  • Up to 10 business days for other ncRNA specific hybridization

At this stage, the RNA Center can provide chromogenic ISH service.  In the near future, we will also provide fluorescence based-ISH and multiple ISH/IHC.

For additional information, including pricing and ordering, please contact Xinna Zhang, Ph.D., Assistant Professor, RNA Center at xzhang12@mdanderson.org.


© 2014 The University of Texas MD Anderson Cancer Center