shRNA and ORFeome Core
The discovery of short hairpin RNAs (shRNAs) suppressing gene expression in mammalian cells enables large-scale loss-of-function screens by using genome-wide shRNA libraries. Genome scale over-expression libraries allow large-scale gain-of-function screens. The ShRNA and ORFeome core makes available to the MDACC research community the human and mouse shRNA libraries, human ORFeome collaboration and LentiORF libraries (www.openbiosystems.com). The core facility can provide individual shRNA plasmids and cDNA clones for MDACC laboratories, or carry out a screening experiment using the libraries.
How to Order
To order services from the facility, use the form and instructions linked here:
- Ordering Instructions (doc)
- Ordering Template (xls)
- Pricing Template (doc)
Libraries
Human and mouse ShRNA libraries: GIPZ Lentiviral shRNAmir libraries targets the entire human and mouse genome with multiple shRNAmir per target gene.
For details: http://www.openbiosystems.com/RNAi/shRNAmir
Libraries/GIPZLentiviralshRNAmir/
Human ORFeome Collaboration: each clone contains a sequence-verified open reading frame (ORF) subcloned into Gateway™ recombinational entry vector.
For details: http://www.openbiosystems.com/GeneExpression/Mammalian/Human/ORFeome
Collaboration/
Precision LentiORFs: an expression-ready library, lentiviral ORFs.
For details: http://www.openbiosystems.com/GeneExpression/Mammalian/Precision
LentiORFs/
Equipment
The ShRNA and ORFeome core will offer a few cutting-edge tools tailored for high-throughput screens: flow cytometry; imaging; Biotek synergy H4 plate reader capable of performing absorbance, fluorescence, luminescence and alpha-screen assays; automated workstations, such as Tecan Evo 100 (tissue culture) and Evo 200 (liquid handling, “cherry-picking” and automated plasmid purification).
Services
- Clone distribution **The shRNA and ORF reagents are available to MDACC researchers ONLY.
- Customized shRNA or ORF libraries
- DNA purification: Facilitated by the Tecan automated workstation, the core will purify DNA in a high throughput manner.
- Lentiviral production
- High-through-put Flow cytometry