Skip to Content

Department Platforms

Reverse Phase Protein Arrays (RPPA)

A high-throughput antibody-based technique with the procedures similar to that of Western blots

  • >1000 samples using > 300 different antibodies


 

 

Single Cell Imaging

ONIX - Nikon TiE - FACSARia II system

  • Generate proteomic Imaging and measuring up to 3 fluorescent reporters or markers in single cells (i.e., protein expression or interactions)
  • Sort single cells based on their fluorescence for further experiments
  • Tracking live mammalian, yeast or bacterial cells over time in up to 3 fluorescence channels plus transmitted light

IN Cell Analyzer 1000

Technology:

  • Automated tool for cell based assays
  • Drug profiling
  • Toxicological studies
  • Gene function

Features:

  • Flexible, modular, integrated, and rapid image acquisition platform for cell based assays.
  • An automated cellular and subcellular imaging system for fast, automated multi-wavelength imaging and analysis in fixed and live cells.
  • Core components including a high-resolution CCD camera, quality optics, laser Autofocus, and a motorized stage.


Genomics

Illumina Microarrays System

Gene Expression Profiling

  • Generate gene expression profiling data from cells and tumor tissues (Illumina platform)
  • Discover potential prognostic or predictive biomarkers
  • Gene expression signatures

Fluidigm

Applications:

  • Gene Expression: High throughput (9,000 qPCR reactions)• Low cost • Compatibility with existing reagents
  • Single-Cell Genomics: Transcriptome Analysis with single cell mRNA sequencing; Gene Expression Profiling with single-cell specific target amplification to validate and routinely measure differential expression of known transcripts
  • Single Nucleotide Polymorphism (SNP) Genotyping: Higher sample throughput;  Outstanding data quality;  Dramatically lower costs
  • Targeted Resequencing: Sequence Large Numbers of Samples across specific regions of interest • Target enrichment • Sample barcoding for multiplexed sequencing • Sequencing library preparation using amplicon tagging
  • Copy Number Variation: High-resolution CNV studies using digital PCR • Simple, fast, and reliable workflow • Standard assays and reagents

Ion Torrent - Proton Semiconductor Sequencing

Features:

  • Ion Personal Genome Machine (PGM™) Sequencer combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data
  • Enable fast, affordable whole-genome sequencing in our lab. Proton-2 chip holds >0.5 billion wells
  • Two-hour run times for 100-base sequencing on the Ion Proton I Chip

Applications:

  • Transcriptome Sequencing
  • Exome Sequencing
  • Targeted DNA/RNA Sequencing
  • Genome Sequencing

  

Sequenom

Mass Spectrometry-based Technology

  • MassARRAY platform for SNP genotyping, mutations, and methylation.  
  • Detection and analysis of circulating cell-free fetal (ccff) nucleic acids (RNA or DNA) from blood sample

Research Areas:

  • Pharmacogenomics
  • Translational Research
  • Cancer Solutions

 

     


© 2014 The University of Texas MD Anderson Cancer Center